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International Journal of Computational Bioinformatics and In Silico Modeling
2013: Volume-2 Issue-4
ISSN: 2320-0634

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ABSTRACT   REFERENCES  
International Journal of Computational Bioinformatics and In Silico Modeling 2(4) 2013: 159-166

Novel mutations in the thymidne kinase gene of ocular herpes simplex virus isolate responsible for acyclovir resistance by in silico analysis


Samson Moses Y1, Malathi J2*, Umashankar V2, Sherlin2 and Madhavan H.N1

1 L & T Microbiology Research Center, Kamal Nayan Bajaj Research Centre, Vision Research Foundation, Sankara Nethralaya, 18, College Road, Chennai- 600 006.
2 Dept of Bioinformatics, Kamal Nayan Bajaj Research Centre, Vision Research Foundation, Sankara Nethralaya, Old No 18, College Road, Chennai- 600 006.

* Corresponding Author

ABSTRACT

Resistance to the commonly used antiviral drugs (namely Acyclovir) is posing a problem among Herpes simplex virus infections. Mutations in the thymidine kinase gene have been identified to be responsible for the resistance expressed by these viruses. In this study we have characterized the phenotypic and genotypic drug resistance of two ocular isolates, which have been further studied by in-silico methods. The phenotypic susceptibility of the isolate was studied by Plaque Reduction Assay method. The Thymidine kinase gene (UL23) of the isolates was amplified and further sequenced to detect mutations. The structure of wild type and mutant type thymidine kinase were elucidated by molecular modeling and the structural deviations between them were studied. The binding energies of Acyclovir with mutant and wild type thymidine kinase were analyzed from docking studies. PRA result showed that the isolate was resistant to 400 µg/ml of the drug. Targeted sequencing results revealed 11 mutations, of which two were novel mutations (Leu43Pro and Gln89Arg.). The structure of mutant TK was in more open conformation compared to wild TK structure. Docking studies infer the greater affinity of Acyclovir binding to wild TK in comparison to mutant TK.


Copyright © 2013 | AIZEON publishers | All rights reserved

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Citation: Samson Moses et al. (2013). Novel mutations in the thymidne kinase gene of ocular herpes simplex virus isolate responsible for acyclovir resistance by in silico analysis. Int J Comput Bioinfo In Silico Model 2(4): 159-166

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